IFN-gamma Receptor (Chain beta) antibody

Catalog No. ABIN967655

The Armenian Hamster Monoclonal anti- antibody has been validated for FACS, IP and WB. It is suitable to detect in samples from Mouse. There is 1 publication available.

Quick Overview for IFN-gamma Receptor (Chain beta) antibody (ABIN967655)

Target: IFN-gamma Receptor

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Clone: MOB-47

Product Details

Binding Specificity: Chain beta

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse IFN-gammaRbeta chain protein

Isotype: IgG

Application Details

Application Notes: Immunofluorescent Staining and Flow Cytometric Analysis: The purified form of MOB-47 (ABIN967655) can be used for the immunofluorescent staining (Less or equal than 1 µg antibody/10e6 cells) and flow cytometric analysis of normal mouse cells or cell lines to measure their expressed levels of IFN-gammaRbeta. An appropriate purified immunoglobulin isotype control is clone A19-3. A three-layer staining protocol is recommended for maximizing the detection IFN-gammaRbeta chains expressed by cells as detailed in the figure legend.
The IFN-gammaRbeta receptor epitope recognized by the MOB-47 antibody is blocked by bound IFN-gamma. Thus, it has been reported that cells which have bound IFN-gamma can be pretreated with dilute acid to remove the bound ligand before they are stained. IP/WB: The MOB-47 antibody has been reported to be useful for the immunoprecipitation and Western blotting of IFN-gammaRbeta chains from lysates of cloned mouse T cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Bach, Szabo, Dighe, Ashkenazi, Aguet, Murphy, Schreiber: "Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1215-8, (1996) (PubMed).

Target Details

Target: IFN-gamma Receptor

Background: The MOB-47 antibody recognizes the 60-65 kD beta chain subunit of the mouse interferon-gamma receptor (IFN-gammaRbeta). The IFN-gamma receptor beta chain is expressed by a variety of normal mouse cell types and cell lines that are responsive to IFN-gamma including T and B lymphocytes, NK cells, monocytes, macrophages, granulocytes and fibroblasts. The IFN-gammaRbeta receptor epitope recognized by the MOB-47 antibody is blocked by bound IFN-gamma. The immunogen used to generate this hybridoma was the purified soluble extracellular domain of the mouse IFN-gammaRbeta chain protein.